This study aimed to prepare some new 2-(1H-tetrazol-5-yl) pyridine derivatives from Pyridine Nitrile as a starting materials and to evaluate the antitubercular activity against Mycobacterium Tuberculosis. The first step involves the reaction of 2-(1H-tetrazol-5-yl) pyridine with ethyl chloroacetate to form ethyl [5-(pyridin-2-yl)-1H-tetrazol-1-yl] acetate (A). In step II it was further treated with hydrazine hydrate to form 2-[5-(pyridin-2-yl)-1H-tetrazol-1-yl] acetohydrazide (B). The compound B was treated with different aromatic aldehyde to form N'-[(E)-substituted phenylmethylidene]-2-[5-(pyridin-2-yl)-1H-tetrazol-1-yl] acetohydrazide (Schiff Bases) (C1–C10). The compounds obtained were identified by spectral data. The antitubercular activity of synthesized compounds were evaluated against Mycobacterium Tuberculosis. The compound C4 and C10 was found to possess potent activity while compounds C2 and C3 shows moderate activity.