Coupling of Zinc of Nanoparticle with Green Matrix of Fish Mucus of Nile tilapia (Oreochromis niloticus)
Mod J Med Biol
The present study was designed to document the antibacterial activity and biochemical composition of the fish skin mucus against different bacterial strains. Nile tilapia was selected for analysis of their mucus sample and the activities of antibacterial against the Gram positive and Gram negative bacteria. Antibacterial potential of fish mucus was evaluated through well diffusion method. Crude mucus extracts showed slightly higher activity of antibacterial activity of Gram negative (Escherichia coli) and Gram positive (Bacillus subtilis) bacteria then the nanoparticle synthesis mucus extract of Nile tilapia. Samples were tested for their hemolytic (0.12 ± 0.01). The fish mucus activities of antioxidants were checked by DPPH (22.64 ± 0.43), reducing power (32.46 ± 0.35), TPC (68.33 ± 1.31), TFC (70.30 ± 2.16) and for biochemical analysis CAT (15.28 ± 0.70), POD (2.19 ± 0.18), SOD (10.05 ± 0.04) and protein estimated (5.09 ± 0.13) was also recorded. Fourier infrared spectroscopy (FTIR) and UV spectra had been used for the characterization of the fish mucus. FTIR results in fish mucus showed the presence of aliphatic primary amines (N-H) and alkenes as a functional group (C=C) at different peaks of spectrum.
Keywords: Fish; Mucus; Nanoparticles; Zinc Oxide