Estimation of CYP3A4*1B Single Nucleotide Polymorphism Using Target-Assembled in-situ Detection by Synthetic DNA-Mounted Excimers

Current Issues

2021 : Volume 1, Issue 1

Author(s) s: Inass A Sadawe, ¹ Nisreen H Meiqal, ¹ Salah M Bensaber, ¹ Abdulhamid R Hamid, ² Massaud Salem Maamar, ³ Anton Hermann ⁴ and Abdul M Gbaj ¹

¹ Department of Medicinal Chemistry , University of Tripoli , Libya

² , Judicial Expertise and Research Centre , Libya

³ Department of Zoology , University of Tripoli , Libya

⁴ Department of Biosciences , University of Salzburg , Austria

Open J Pharma Sci

Article Type : Research Article

CYP3A4*1B is a single nucleotide polymorphism of CYP3A4 and is associated with prostate cancer which exhibits higher nifedipine oxidase activity in liver. This research provides details of the effects of structural variation and medium effects for the recently reported split-oligonucleotide (tandem) probe system for excimers-based fluorescence detection of DNA. In this approach the detection system is split at a molecular level into signal-silent components, which must be assembled correctly into a specific 3-dimensional structure to ensure close proximity of the excimer partners and the consequent excimer fluorescence emission on excitation. The model system consists of two 11-mer oligonucleotides, complementary to adjacent sites of a 22-mer DNA target. Each oligonucleotide probe is equipped with functions able to form an excimer on correct, contiguous hybridization. The extremely rigorous structural demands for excimer formation and emission required careful structural design of partners for excimer formation, which are here described. This study demonstrates that the excimer formed emitted at ~480 nm with a large Stokes shift (~130-140 nm).